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Millipore Protein A Agarose/Salmon Sperm Dna, 2.5 Ml
Millipore Protein A Agarose/Salmon Sperm Dna, 2.5 Ml
List Price$489.29Your Price$489.29
Millipore Recombinant Protein A Covalently Bound To Agarose Beads For Use In Chromatin Immunoprecipitations (Chip Assays). Sonicated Salmon Sperm Dna Is Included To Block Non-Specific - Mill (Additional S&H or Hazmat Fees May Apply)
Achieve first time ChIP success with EZ-ChIP Assays
Description
Catalogue Number
16-157
Brand Family
Upstate
Trade Name
Upstate
Description
Protein A Agarose/Salmon Sperm DNA, 2.5 mL
Overview
Protein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.
Alternate Names
ChIP agarose beads
ChIP agaraose A beads
Background Information
Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
Recombinant protein A covalently bound to agarose beads for use in chromatin immunoprecipitations (ChIP assays). Sonicated salmon sperm DNA is included to block non-specific DNA binding sites on agarose beads. Every lot qualified using an Acetyl Histone H4 and H3 ChIP assay.
Application Notes
Suitable for use in chromatin immunoprecipitation (ChIPs) assays
Product Usage Statements
Quality Assurance
Routinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
1 year at 4°C
Packaging Information
Material Size
2.5 mL
Material Package
2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
