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Millipore Protein G Agarose/Salmon Sperm Dna, 2.5 Ml
Millipore Protein G Agarose/Salmon Sperm Dna, 2.5 Ml
List Price$489.29Your Price$489.29
Millipore Recombinant Protein G Covalently Bound To Agarose Beads By Cyanogen Bromide Linkage For Use In Chromatin Immunoprecipitations (Chip Assays). Sonicated Salmon Sperm Dna Is - Mill (Additional S&H or Hazmat Fees May Apply)
Achieve first time ChIP success with EZ-ChIP Assays
Description
Catalogue Number
16-201
Brand Family
Upstate
Trade Name
Upstate
Description
Protein G Agarose/Salmon Sperm DNA, 2.5 mL
Overview
Recombinant Protein G covalently bound to agarose by cyanogen bromide linkage. Sonicated salmon sperm DNA blocks non-specific DNA binding sites on protein G agarose when collecting immunocomplexes for chromatin immunoprecipitations (ChIP assays).
Alternate Names
ChIP agarose beads
ChIP agaraose G beads
Background Information
Recombinant protein G has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein G on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
Product Information
Presentation
2.5 mL packed beads containing 1 mg sonicated salmon sperm DNA, 2.5 mg BSA and approximately 5 mg recombinant Protein G. Provided as a 50% gel slurry for a final volume of 5 mL per vial. Suspended in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, containing 0.05% sodium azide. Liquid suspension.
Quality Level
MQ100
Applications
Application
Recombinant Protein G covalently bound to agarose beads by cyanogen bromide linkage for use in chromatin immunoprecipitations (ChIP assays). Sonicated salmon sperm DNA is included to block non-specific DNA binding sites on agarose beads. Every lot qualified using an Acetyl Histone H3 ChIP assay.
Product Usage Statements
Quality Assurance
Routinely evaluated in Chromatin Immunoprecipitation for use to pre-clear the supernatant from sodium butyrate treated HeLa cells and subsequent immunoprecipitation of DNA cross-linked to acetylated histones
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
1 year at 4°C from date of shipment.
Packaging Information
Material Size
2.5 mL
Material Package
2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
