Key Specifications Table
| Key Applications |
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| KA |
| Description | |
|---|---|
| Catalogue Number | 20-131 |
| Brand Family | Upstate |
| Trade Name |
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| Description | PKC Substrate Cocktail |
| Product Information | |
|---|---|
| Presentation | 20mM MOPS, pH 7.2, 25mM β-glycerophosphate, 1mM sodium orthovanadate, 1mM dithiothreitol, 1mM CaCl2. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Key Applications |
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| Application Notes | For use as a substrate in PKC kinase assays. |
| Biological Information | |
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| Entrez Gene Number |
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| Entrez Gene Summary | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity). SIZE: 672 amino acids; 76764 Da SUBUNIT: Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine. SIMILARITY: SwissProt: P17252 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated in a kinase assay. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 1 year at -20°C |
| Packaging Information | |
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| Material Size | 1 mL |