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Poly-D-Lysine promotes the adhesion of tissues/sections to the culture vessel. The polycationic property of the molecule allows interaction with anionic sites on the tissue/cells promoting effective attachment to the growing surface. The molecular weight of Poly-D-Lysine is >300,000 Da.
Alternate Names
poly lysine
Product Information
Presentation
Liquid. 20ml in sterile water.
Quality Level
MQ100
Applications
Application
The Poly-D-Lysine solution promotes the adhesion of tissues/sections to the culture vessel.
Key Applications
Cell Culture
Application Notes
The level of coating will vary for different species and types of cells. In general final coating concentrations from 3-10μg/cm(2) of surface area will typically supply sufficent coatings for most cell types.
Surface area of plates: (pi)(Radius squared) in cm(2)
In partical terms working dilutions of 50μg/mL to 100μg/mL are usually adequate.
Dilute poly-D-lysine in PBS, water, or 0.1M borate buffer {prepared by adding 1.24 g boric acid and 1.9 g sodium tetraborate in 400 ml water, pH 8.5} to final working concentration.
Plates or flasks are coated for 3 hours to overnight, and up to 3 days if stored at 4C. Best results are usually with just over night coatings.
Remove poly-d-lysine solution and rinse with sterile PBS or water 1-2X. Do not scrape the bottom of the coated plates or flasks.
Use plates immediately or store with PBS for up to 5 days.
Poly-D-lysine coating methods will vary slightly from laboratory to laboratory, please use the method as a general guide.
Biological Information
Media Form
Liquid
Stem Cell Type
Human Embryonic Stem Cells
Mesenchymal Stem Cells
Neural Stem Cells
Hematopoietic Stem Cells
Epithelial Cells
Pancreatic Stem Cells
Induced Pluripotent Stem Cells
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
