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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | DB, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10050 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-phospho-ITCH (pT222) Antibody |
| Alternate Names |
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| Background Information | ITCH is an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. ITCH regulates the transcriptional activity of several transcription factors including the hematopoietic transcription factor NF-E2, and may play an important role in the regulation of immune response. ITCH down-regulates Epstein-Barr virus LMP2A activity in B cell signaling, and also interacts with NOTCH1, OCLN, JUN, and JUNB. ITCH has a wide tissue distribution and is localized within the nucleus and cytoplasm. Two ITCH splice variants have been described; an approximately 103 kDa form as well as a truncated form (~98 kDa) lacking 42 amino acids in the N-terminal half of the protein. ITCH contains a MAP Kinase binding site and its activity is modulated by phosphorylation at multiple threonine residues by JNK1. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in Tris-citrate/phosphate buffer, pH 7-8, containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-phospho-ITCH (pT222) Antibody is validated for use in DB, WB for the detection of phospho-ITCH (pT222). |
| Key Applications |
|
| Application Notes | Western Blot: Recommended Dilution 1 - 5 μg/mL Dot Blot |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to a portion of human ITCH encompassing the phosphorylated Threonine at position 222 (PPPpTPRRP). |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Human ITCH phosphorylated on threonine 222 (T222). |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells. |
| Gene Symbol |
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| Modifications |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q96J02 # E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. SIZE: 903 amino acids; 102803 Da SUBUNIT: Interacts via its WW domains with DRPLA, NFE2 and CBLC. Interacts with Epstein-Barr virus LMP2A. Interacts with NOTCH1, OCLN, JUN and JUNB. Interacts with NDFIP1 in vitro (By similarity). SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). TISSUE SPECIFICITY: Widely expressed. PTM: Phosphorylated on tyrosine residues. SIMILARITY: SwissProt: Q96J02 ## Contains 1 C2 domain. & Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. & Contains 4 WW domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C for up to 12 months from date of receipt |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |