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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Chp, Rhesus Macaque, Ca | ICC, IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10181 |
| Description | Anti-Pumilio-2 (PUM2) Antibody |
| Alternate Names |
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| Background Information | PUM2 is a member of the Puf family of RNA-binding proteins, which are believed to play a role in the regulation of posttranscriptional gene expression. Members of the Puf family of RNA binding proteins function as repressors of translation when bound to the 3-untranslated region of RNA transcripts. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Pumilio-2 (PUM2) using this Anti-Pumilio-2 (PUM2) Antibody validated for use in IC, IH & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:400 dilution from a previous lot detected Pumilio-2 in human testis and teratoma tissue. Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Pumilio-2 in human and mouse embryonic stem cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the N-terminus of human Pumilio-2. |
| Epitope | N-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes Pumilio-2 near the N-terminus. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human and mouse. Predicted to react with chimpanzee, rhesus monkey, and canine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts. SUBUNIT STRUCTURE: Homodimer; homodimerizes in vitro. Interacts with DAZ, DAZL and NANOS1 via its pumilio repeats. Binds to a RNA consensus sequence, that is related to the Nanos Response Element (NRE), a 16 bp sequence found in the 3-UTR of the Drosophila hb mRNA. Also binds to the NRE. SUBCELLULAR LOCATION: Cytoplasm Probable. TISSUE SPECIFICITY: Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested. INDUCTION: Down-regulated in keratinocytes upon UV B radiation. DOMAIN: The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half donut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs By similarity. SEQUENCE SIMILARITIES: Contains 1 PUM-HD domain. Contains 8 pumilio repeats. |
| Molecular Weight | ~120 kDa observed. Non specific band may be observed at 48 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on mouse embryonic stem cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected Pumilio-2 (PUM2) in 10 µg of mouse embryonic stem cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |