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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IF, IH(P), IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1031 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Aggrecan Antibody |
| Alternate Names |
|
| Background Information | Aggregating chondroitin sulfate proteoglycan or aggrecan, is one of the most studied proteoglycans. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Rabbit polyclonal antigen affinity purified antibody in PBS. Anti-GST has not been adsorbed. Liquid. Note: Aggrecan is present in articular cartilage, brain, heart, and other tissues. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Aggrecan Antibody is an antibody against Aggrecan for use in IF, IH(P), IP & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry(Paraffin): A previous lot of this antibody was used at 10 μg/mL. See note under Protocol regarding triton X-100 treatments. Immunoprecipitation: A previous lot of this antibody was used at 5 μg/ 500 μL of cell membrane extract in PBS with inhibitors and >0.5% triton X-100. |
| Biological Information | |
|---|---|
| Immunogen | GST fusion protein containing amino acids 1177-1326 of mouse aggrecan. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | AB1031 is specific for mouse aggrecan; other species have not been tested. |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Expected to cross react with Human and rat. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on Mouse Brain lysates. Western Blot Analysis: 1:500 dilution of this lot detected AGGRECAN on 10 μg of Mouse Brain lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |