Name: Millipore Anti-Aggrecan Antibody
SKU: MILL-AB1031
Price: 583.86 USD

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Key Specifications Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	IF, IH(P), IP, WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	AB1031
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Aggrecan Antibody
Alternate Names	Cartilage-specific proteoglycan core protein Cartilage-specific proteoglycan core protein aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122) aggrecan proteoglycan aggrecan proteoglycan large aggregating proteoglycan
Background Information	Aggregating chondroitin sulfate proteoglycan or aggrecan, is one of the most studied proteoglycans. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight.

Product Information
Format	Affinity Purified
Control	Cartilage, neural tube, and brain tissue.
Presentation	Rabbit polyclonal antigen affinity purified antibody in PBS. Anti-GST has not been adsorbed. Liquid. Note: Aggrecan is present in articular cartilage, brain, heart, and other tissues.
Quality Level	MQ100

Applications
Application	Anti-Aggrecan Antibody is an antibody against Aggrecan for use in IF, IH(P), IP & WB.
Key Applications	Immunofluorescence Immunohistochemistry (Paraffin) Immunoprecipitation Western Blotting
Application Notes	Immunohistochemistry(Paraffin): A previous lot of this antibody was used at 10 μg/mL. See note under Protocol regarding triton X-100 treatments. Immunoprecipitation: A previous lot of this antibody was used at 5 μg/ 500 μL of cell membrane extract in PBS with inhibitors and >0.5% triton X-100.

Biological Information
Immunogen	GST fusion protein containing amino acids 1177-1326 of mouse aggrecan.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	AB1031 is specific for mouse aggrecan; other species have not been tested.
Species Reactivity	Mouse
Species Reactivity Note	Mouse. Expected to cross react with Human and rat.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001126
Entrez Gene Summary	This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.
Gene Symbol	ACAN MSK16 SEDK CSPCP AGCAN AGC1 CSPG1 CSPGCP
Purification Method	ImmunoAffinity Purified
UniProt Number	P16112
UniProt Summary	FUNCTION: SwissProt: P16112 # This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. SIZE: 2415 amino acids; 250193 Da SUBUNIT: Interacts with FBLN1 (By similarity). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity). TISSUE SPECIFICITY: Restricted to cartilages. DEVELOPMENTAL STAGE: Expression was detected in chondrocytes throughout the developing skeleton. DOMAIN: SwissProt: P16112 Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3. PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase. DISEASE: SwissProt: P16112 # Defects in AGC1 are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]. Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. SIMILARITY: Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 4 Link domains. & Contains 1 Sushi (CCP/SCR) domain.

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on Mouse Brain lysates. Western Blot Analysis: 1:500 dilution of this lot detected AGGRECAN on 10 μg of Mouse Brain lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	50 µg

More Information

SKU

MILL-AB1031

Supplier Part Number

AB1031

UNSPSC

12352203

Manufacturer

Millipore

ProductLine

Mill

Qty

MinOrderQty

Weight

10.000000

Lead Time

Hazardous

Energy Star

Green

Controlled

NETA PART NO.	Quantity

Millipore Anti-Aggrecan Antibody

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Key Specifications Table

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