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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IP, WB, IH(P) | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1032 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Versican Antibody, a.a. 535-598 of mouse versican |
| Alternate Names |
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| Background Information | Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan., Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Immunoglobulin was purified by affinity chromatography and is presented in PBS with no preservative Anti-GST has not been adsorbed. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Versican Antibody, a.a. 535-598 of mouse versican is validated for use in IP, WB, IH(P) for the detection of Versican. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (formalin fixed, paraffin embedded): 10 μg/mL. Tissues must be treated with Chondroitinase before staining {0.5U/mL in 100mM Tris-HCL pH 7.2-7.4, 30 minutes at room temperature} Then block and stain. Western blotting: 0.5 μg/mL Immunoprecipitation Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | GST fusion protein containing amino acids 535-598 of mouse versican. |
| Epitope | a.a. 535-598 of mouse versican |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Mouse versican GAG alpha domain |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P13611 # May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. SIZE: 3396 amino acids; 372820 Da SUBUNIT: Interacts with FBLN1 (By similarity). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. TISSUE SPECIFICITY: Cerebral white matter. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas; isoform V2 is restricted to normal brain and gliomas; isoform V3 is found in all these tissues except medulloblastomas. DEVELOPMENTAL STAGE: Disappears after the cartilage development. DISEASE: SwissProt: P13611 # Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN. SIMILARITY: SwissProt: P13611 ## Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 2 EGF-like domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 2 Link domains. & Contains 1 Sushi (CCP/SCR) domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |