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PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis
Product Information
Format
Purified
Control
Western Blot : Mouse brain lysate
Presentation
Purified in PBS with 0.09% NaN3.
Quality Level
MQ100
Applications
Application
Anti-Puma BH3 domain Antibody is an antibody against Puma BH3 domain for use in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
This antibody was developed against a synthetic peptide selected from the C-terminal region of human Puma.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
The antibody reacts with the 21 kDa Puma BH3 domain protein.
Species Reactivity
Mouse
Species Reactivity Note
Reacts with mouse. Other species have not been tested.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_055232
Gene Symbol
JFY-1
JFY1
PUMA
Purification Method
Protein G Purified
UniProt Number
Q9BXH1
UniProt Summary
FUNCTION: Essential mediator of p53-dependent and p53-independent apoptosis. SUBUNIT STRUCTURE: Interacts with MCL1 and BCL2A1 By similarity. Interacts with BCL2 and BCL2L1/BCL-XL. SUBCELLULAR LOCATION: Mitochondrion. Note= Localized to the mitochondria in order to induce cytochrome c release. TISSUE SPECIFICTY: Ubiquitously expressed. INDUCTION: By DNA damage, glucocorticoid treatment, growth factor deprivation and p53. SEQUENCE SIMILARITIES: Belongs to the Bcl-2 family.
Molecular Weight
21 kDa
Product Usage Statements
Quality Assurance
Western Blot Analysis: 1:250 dilution of this antibody detected Puma BH3 domain on mouse brain lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at 2-8°C for 6 months from date of receipt. For long term storage, store at -20°C
