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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10423 |
| Description | Anti-Phospho- Bad (Ser75) Antibody |
| Alternate Names |
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| Background Information | Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified immunoglobulins in PBS with 0.09% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Phospho-Bad (Ser75) Antibody detects level of Phospho-Bad (Ser75) & has been published & validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | This antibody is generated in rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS75 of human Bad. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody reacts with the 18 kDa phospho-Bad (Ser75) protein. |
| Species Reactivity |
|
| Species Reactivity Note | Reacts with mouse and rat. Other species have not been tested. Predicted to react with human based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq] |
| Gene Symbol |
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| Modifications |
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| Purification Method | Protein G followed by IAP |
| UniProt Number |
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| UniProt Summary | FUNCTION: Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 By similarity. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. SUBUNIT STRUCTURE: Forms heterodimers with the anti-apoptotic proteins, Bcl-X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 By similarity. The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins By similarity. SUBCELLULAR LOCATION: Mitochondrion outer membrane. Cytoplasm. Note= Upon phosphorylation, locates to the cytoplasm. TISSUE SPECIFICTY: Expressed in a wide variety of tissues. DOMAIN: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. PTM: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation By similarity. SEQUENCE SIMILARITIES: Belongs to the Bcl-2 family. SEQUENCE CAUTION: The sequence AAB36516.1 differs from that shown. Reason: Frameshift at positions 64 and 91. |
| Molecular Weight | 18 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Western Blot Analysis: 1:250 dilution of this antibody detected phospho-Bad (Ser75) in lysate from LPS-stimulated RAW264.7 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at 2-8°C for 6 months from date of receipt. For long term storage, store at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 400 µl |