Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, Rhesus Macaque, Ca | WB, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10518 |
| Replaces | AB1656 |
| Description | Anti-DARPP-32 Antibody |
| Alternate Names |
|
| Background Information | Dopamine- and cAMP-regulated neuronal phosphoprotein (DARP-32) is a cytoplasmic member of the protein phosphatase inhibitor 1 family. DARP-32 is a multifunctional protein kinase and protein phosphatase regulator, depending on the site of its activation. It possesses at least four sites that can be phosphorylated. Phosphorylation by PKA at Thr34 stimulates DARP-32 to act as a protein-phosphatase-1 (PP-1) inhibitor. However, phosphorylation at Thr75 will inhibit Thr34 phosphorylation. This selective, bidirectional regulation allows DARPP-32 to have a wide range of influence on downstream physiological effectors such as: ion pumps, neurotransmitter receptors, voltage-gate ion channels, and transcriptional factors. Studies suggest that it may also have a role in the Circadian System photic pathway. Overexpression of DARPP-32 has been linked with antiapoptotic mechanisms of cancer cells and has been observed in breast, colon, esophagus, gastric, and prostate cancer tissues. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-DARPP-32 Antibody is an antibody against DARPP-32 for use in WB & IP. |
| Key Applications |
|
| Application Notes | Western Blot (Snap i.d.) Analysis: 1 µg/mL of this antibody from a previous lot detected DARPP-32 on 10 µg of rat brain tissue lysate. Immunoprecipitaion Analysis: 10 µg of this antibody from a previous lot immunoprecipitated DARPP-32 from rat brain RIPA lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to mouse DARPP-32 near the N-terminus. |
| Epitope | Near N-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes DARPP-32 near the N-terminus. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with rat and mouse. Predicted to react with rhesus monkey and canine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Inhibitor of protein-phosphatase 1. SUBCELLULAR LOCATION: Cytoplasm. PTM: Dopamine- and cyclic AMP-regulated neuronal phosphoprotein (By similarity). Phosphorylation of Thr-34 is required for activity (By similarity). SEQUENCE SIMILARITIES: Belongs to the protein phosphatase inhibitor 1 family. |
| Molecular Weight | ~ 32 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on rat brain tissue lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected DARPP-32 on 10 µg of rat brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |