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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H | WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10532 |
| Description | Anti-GABA(A) receptor subunit gamma-2 Antibody |
| Alternate Names |
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| Background Information | GABA(A) receptor subunit gamma-2 is a protein belonging to a subclass of GABA receptors known as GABA (A). GABA (A) receptors are pentameric chloride channels composed of subunits belonging to different homology classes, i.e. α, β, γ, δ, θ, ε, π, and act as receptors for GABA, a crucial inhibitory neurotransmitter localized within the brain. GABA (A) gamma 2 subunit consists of 5 cysteine residues and carries the binding site for benzodiazepine. The subunit functions to promote receptor maintenance and clustering at the synapse, interacts with GABA receptor associated proteins such as GODZ and GABAWRAP, affects GABA (A) receptor conductance and kinetics, and is a contributing component to receptors participating in both phasic and tonic inhibition. Several mutations in the GABA(A) receptor subunit gamma-2 expression have been linked to epileptic syndromes that result in fibrile seizures. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit polyclonal serum containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-GABA(A) receptor subunit gamma-2 Antibody is validated for use in WB for the detection of GABA(A) receptor subunit gamma-2. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to rat GABA(A) receptor subunit gamma-2. |
| Host | Rabbit |
| Specificity | This antibody recognizes GABA(A) receptor subunit gamma-2. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Subunit of GABA receptors, which bind benzodiazepines and act as inhibitory ion channels in the brain [RGD]. |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. SUBUNIT STRUCTURE: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. SUBCELLULAR LOCATION: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. PTM: Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability (By similarity). MISCELLANEOUS: This subunit carries the benzodiazepine binding site. SEQUENCE SIMLARITIES: Belongs to the ligand-gated ionic channel (TC 1.A.9) family. (View classification) |
| Molecular Weight | ~60 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in PC12 cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected GABA(A) receptor subunit gamma-2 on 10 µg of PC12 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |