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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IH(P), WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10554 |
| Description | Anti-Tbr1 Antibody |
| Alternate Names |
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| Background Information | T-box brain protein 1 (UniProt: Q64336; also known as T-brain-1, TBR-1, TES-56) is encoded by the Tbr1 gene (Gene ID: 21375) in murine species. Tbr1 is a homodimeric nuclear protein that serves as a transcriptional repressor and is involved in multiple aspects of cortical development, including neuronal migration, laminar and areal identity, and axonal projection. As transcriptional repressor of FEZ Family Zinc Finger 2 (FEZF2), it blocks the formation of the corticospinal (CS) tract from layer 6 projection neurons, thereby restricting the origin of CS axons specifically to layer 5 neurons. Its T-box DNA binding domain is localized in amino acids 213-393. Its expression is observed in developing and adult cerebral cortex and in olfactory bulbs. It is detected around day 10 of embryonic development in the preplate and at day 12.5 in the cortical plate. It is highly expressed in glutamatergic early-born cortical neurons. Tbr1 null mice exhibit severe defects of frontal cortex and layer 6 differentiation. (Ref.: Han, W., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(7); 3041-3046; Bedogni, F., et al (2010). Proc. Natl Acad. Sci. USA. 107(29); 13129-13134; Hevner, RF., et al. (2001). Neuron. 29(2); 353-366). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Tbr1, Cat. No. AB10554, is a rabbit polyclonal antibody that detects Tbr1 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Tested Applications Western Blotting Analysis: A 1:500 dilution from a representative lot detected Tbr1 in Mouse fetal brain tissue lysate. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of mouse T-box brain protein 1 (Tbr1). |
| Epitope | This antibody recognizes Tbr1 near the N-terminus. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects T-box brain protein 1 (Tbr1). |
| Species Reactivity |
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| Species Reactivity Note | Mouse. Predicted to react with Horse, Porcine, Canine, Rat, Bovine, Opossum based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Probable transcriptional regulator involved in developmental processes. TBR1 is required for normal brain development. SUBCELLULAR LOCATION: Nucleus (Potential). TISSUE SPECIFICTY: Expressed in specific lamina in the developing and adult brain. DEVELOPMENTAL STAGE: First detected around day 10 of embryonic development in the preplate, at day 12.5, in the cortical plate and intermediate zone, and from day 16.5 to 18.5, in a rostro-caudal gradient in the subplate. In the thalamus, expression is first observed at postnatal stage, P7, and weak expression continues in later postnatal and adult stages. SEQUENCE SIMILARITIES: Contains 1 T-box DNA-binding domain. |
| Molecular Weight | ~76 kDa observed; 73.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in Mouse brain tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution of this antibody detected Tbr1 in Mouse cerebral cortex and cerebellum tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |