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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, PO | IHC, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1254 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cytochrome P450 Enzyme CYP3A4 Antibody |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit antiSerum. Liquid containing no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cytochrome P450 Enzyme CYP3A4 Antibody detects level of Cytochrome P450 Enzyme CYP3A4 & has been published & validated for use in IH & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:1000-1:2000: Using microsomal membrane fractions, detects a band at ~54kDa; other bands may be present as well. Antibody has been successfully used to detect CYP3A4 on LICOR fluorescent westerns as well. Immunohistochemistry: 10% formalin fixed, paraffin embedded tissues 1:200-1:400, microwave-citric acid antigen recovery is suggested. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | A synthetic peptide containing the C-terminal sequence of TVSGA from human CYP3A4. The peptide was coupled to KLH. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The rabbit polyclonal serum detects CYP3A4 in humans and rats; by sequence homology of the immunogen used, cross reactivity to CYP3A7, 3A6v4;3A2v1, 3A8, 3A9 may be expected but is untested at this time. |
| Species Reactivity |
|
| Species Reactivity Note | Expects reactivity with rat and other mammals based on homology, but this is untested. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene, CYP3A4, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P08684 # Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide. SIZE: 503 amino acids; 57343 Da SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein. TISSUE SPECIFICITY: Expressed in prostate and liver. SIMILARITY: SwissProt: P08684 ## Belongs to the cytochrome P450 family. |
| Molecular Weight | 57 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |