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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, Mk, Gp, Ht, Gr | IH(P), ICC, IHC, IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1506 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Glutamate Receptor 2 & 3 Antibody |
| Alternate Names |
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| Background Information | Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcategorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified rabbit polyclonal serum lyophilized from buffer containing 0.01M phosphate, 0.09M NaCl, pH 7.2. 1% BSA and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Glutamate Receptor 2 & 3 Antibody is validated for use in IH(P), IC, IH, IP, WB for the detection of Glutamate Receptor 2 & 3. |
| Key Applications |
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| Application Notes | Immunohistochemistry: A previous lot was used on paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations used were 1-3 µg/mL. Immunoprecipitation: When linked to immobilized Protein A, a previous lot of this antibody immunoprecipitated detergent-solubilized receptor from brain or transfected cells. |
| Biological Information | |
|---|---|
| Immunogen | Carboxy terminus peptide of rat GluR2 conjugated to BSA with glutaraldehyde (EGYNVYGIESVKI). |
| Epitope | C-terminus |
| Host | Rabbit |
| Specificity | It recognizes both GluR2 and GluR3 which have nearly identical carboxy terminal sequences. Western blot analysis of rat brain shows two poorly separated bands which comigrate with GluR2 and GluR3 expressed in transfected cells. GluR2 migrates slightly ahead of GluR3. No cross-reaction with GluR1 or GluR4. The antibody appears to recognize GluR 2 & 3 in several other species, but has not been extensively characterized in these cases. |
| Species Reactivity |
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| Species Reactivity Note | Mouse. Expected to cross-react with human, rat, monkey, gerbil, guinea pig, and hamster. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) encodes isoform 1 (also known as flip isoform). RNA editing (CAG->CGG) changes aa Gln607Arg. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript matching the reference genomic sequence was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
| Gene Symbol |
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| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P42262 # Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. SIZE: 883 amino acids; 98821 Da SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4. Interacts with PRKCABP, GRIP1 and GRIP2 (By similarity). SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity). SIMILARITY: SwissProt: P42262 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family. MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate. |
| Molecular Weight | 108 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on rat brain lysate. Western Blot Analysis: 1:1000 dilution of this antibody detected glutamate receptor 2 & 3 on 10 μg of rat brain lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain lyophilized material at +2-8°C or -20°C for up to 6 months after date of receipt. Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks at +2-8°C and reused repeatedly. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |