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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M | IH(P), ICC, IHC, IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1508 |
| Replaces | 06-308 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Glutamate Receptor 4 Antibody |
| Alternate Names |
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| Background Information | Glutamate receptor 4 (UniProt: P48058; also known as GluR-4, GluR4, AMPA-selective glutamate receptor 4, GluR-D, Glutamate receptor ionotropic AMPA 4, GluA4) is encoded by the GRIA4 (also known as GLUR4) gene (Gene ID: 2893) in human. The postsynaptic actions of glutamate are mediated by a variety of receptors that are named according to their selective agonists. GluR-4 is a multi-pass membrane protein that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. It displays greater affinity for AMPA. GluR-4 is synthesized with a signal peptide (aa 1-20), which is cleaved off in the mature form. GluR-4 can exist as a homotetramer or heterotetramer of pore-forming glutamate receptor subunits, tetramers can also form by the dimerization of dimers. GluR-4 is generally palmitoylated and becomes de-palmitoylated upon glutamate stimulation. Palmitoylation on Cys 611 leads to its Golgi retention and reduced cell surface expression. However, palmitoylation at Cys 837 does not affect cell surface expression, but can regulate stimulation-dependent endocytosis. GluR-4 can undergo phosphorylation at Serine 862 by PKC gamma and this phosphorylation increases plasma membrane-associated GRI4 expression. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Glutamate Receptor 4 Antibody is validated for use in IH(P), IC, IH, IP, WB for the detection of Glutamate Receptor 4. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: Dilutions of 1:10 – 1:500 were tested on rat brain lysate. Can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Suggested concentrations start at 1-3 μg/mL. Western blot analysis can be done at final concentrations of 1-3 μg/mL. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic linear peptide. |
| Epitope | C-Terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Cat. # AB1508 recognizes C-Terminus of GluR4. |
| Species Reactivity |
|
| Species Reactivity Note | The immunogen sequence shares 100% conservation with chicken, mouse, rat, oppossum, human, chimp, and monkey. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. |
| Gene Symbol |
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| Purification Method | Antigen Affinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P48058 # Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. SIZE: 902 amino acids; 100810 Da SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with EPB41L1 via its C-terminus (By similarity). SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity). SIMILARITY: SwissProt: P48058 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family. MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate. |
| Molecular Weight | Approx. 100 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse and rat brain membrane. Western Blotting Analysis: A 1:500 dilution of this antibody detected Glutamate Receptor 4 in mouse and rat brain membrane. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |