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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | ELISA, ICC, IHC, IH(P), IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1543P |
| Replaces | MAB355 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Synapsin I Antibody |
| Alternate Names |
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| Background Information | The synapsins are a family of proteins that have long been implicated in the regulation of neurotransmitter release at synapses. Specifically, they are thought to be involved in regulating the number of synaptic vesicles available for release via exocytosis at any one time. Synapsins are encoded by three different genes, synapsin I, II and III, and different neuron terminals will encode different amounts of these; synapsin will make up 1% of total brain protein at any one time. Current studies suggest the following hypothesis for the role of synapsin: synapsins bind synaptic vesicles to components of the cytoskeleton which prevents them from migrating to the presynaptic membrane and releasing transmitter. During an action potential, synapsins are phosphorylated by Ca2+/calmodulin-dependent protein kinase II, releasing the synaptic vesicles and allowing them to move to the membrane and release their neurotransmitter. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified rabbit polyclonal in buffer lyophilized from 5 mM ammonium bicarbonate. Reconstitute with 50 μL of PBS. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Synapsin I Antibody is validated for use in ELISA, IC, IH, IH(P), IP, WB for the detection of Synapsin I. |
| Key Applications |
|
| Application Notes | Western blotting: 1:200-1:1,000 dilution of a previous lot was used. Immunocytochemistry: 1:500-1:2,000 dilution of a previous lot was used. Immunoprecipitation: 1 μg of a previous lot immunoprecipitated all of the synapsin I from a SDS homogenate of 200 μg of rat brain protein. Note: The above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling. Immunohistochemistry: 1:500-1:2,500 ELISA: 1:2,500-1:10,00 Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synapsin I (mixture of Ia & Ib) purified from bovine brain. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Synapsin I (Mr of 80,000 and 77,000). Synapsin is located only in brain nerve terminal and is an excellent marker for synapes. Immunolabeling is blocked by preadsorption of the antibody with synapsin I. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P17600 # Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. SIZE: 705 amino acids; 74111 Da SUBUNIT: Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1 (By similarity). SUBCELLULAR LOCATION: Cell junction, synapse. PTM: Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: SwissProt: P17600 # Defects in SYN1 are a cause of X-linked recessive epilepsy associated with variable learning disabilities and behavior disorders [MIM:300491]. SIMILARITY: SwissProt: P17600 ## Belongs to the synapsin family. |
| Molecular Weight | 77 & 80 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Immunohistochemistry(paraffin): Synapsin I (AB1543P) representative staining pattern/morphology in rat hippocampal neurons. Tissue was pretreated with Citrate pH 6.0, antigen retrieval. This lot of antibody was diluted to 1:1000, IHC-Select® reagents used with HRP-DAB. Immunoreactivity is seen directly associated with terminal end of neuronal cell body. IHC-Paraffin Staining With Epitope Retrieval: Rat Hippocampus |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |