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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, F | IHC, IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1555P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-NMDAR2A Antibody |
| Alternate Names |
|
| Background Information | NMDA (N-methyl-D-aspartate) receptors are a class of ionotropic glutamate receptors. The NMDAR channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal lyophilized from ammonium bicarbonate (5 mM) so some residual salt may also be present. Reconstitute with 50 μL of PBS. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NMDAR2A Antibody is an antibody against NMDAR2A for use in IH, IP & WB. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: 3 μg of a previous lot was used to (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2A in 200 μg of rat brain. Immunohistochemistry: A previous lot of this antibody was used at 1:1,000-1:2,000 dilution. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | C-Terminal Fusion Protein of rat NMDAR2A, aa 1253-1391. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | NMDAR2A. By Western blot it recognizes a 180 kDa band in rat brain membranes. Does not react with NMDAR2B or NMDAR2C. Immunolabeling is blocked by preadsorption of antibody with the immunogen. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of AIP1, INADL and DLG4 By similarity. Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10) family. [View classification] |
| Molecular Weight | 180 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on Rat Brain Microsomal lysates. Western Blot Analysis: 1:500 dilution of this lot detected NMDAR2A on 10 μg of Rat Brain Microsomal lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Lyophilized material stable for 1 year at -20°C from date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up 6 months. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |