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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Gp, R, Rb | IHC, WB | Sh | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1582 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Choline Acetyltransferase Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Choline Acetyltransferase Antibody is validated for use in IH, WB for the detection of Choline Acetyltransferase. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:1,000-1:2,000 Immunoblotting Optimal working dilutions must be determined by end user. IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1582 STAINING OF GUINEA PIG ILEUM 1) Pin the ileum out flat and tight. 2) Fix overnight at 4°C in modified Zambonis (2% paraformaldehyde with 15% saturated picric acid in phosphate buffered saline). 3) Clear in DMSO, 100%, 3 times for 10 minutes each, rinse in PBS. 4) Dissect off ALL circular muscle. 5) Preincubate with 10% normal donkey serum. 6) Incubate in anti-ChAT (AB1582) diluted 1:1,000-1,2000 with 10% normal donkey serum over night at 4°C on a shaker. 7) Incubate with Donkey anti-Sheep IgG-Cy3 labeled secondary antibody (Catalog Number AP184C) at a dilution of 1:200 for 2 hours. IMPORTANT NOTE: It is essential to remove ALL circular muscle. |
| Biological Information | |
|---|---|
| Immunogen | A 22 amino acid peptide from porcine ChAT, coupled to KLH. The sequence is H-GLFSSYRLPGHTQDTLVAQKSS-NH2. |
| Host | Sheep |
| Specificity | Choline Acetyltransferase (ChAT). Will stain cholinergic neurons in rat, guinea-pig and rabbit central and peripheral nervous systems. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5 untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. SIZE: 748 amino acids; 82568 Da DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive. SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |