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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | IHC, IH(P), WB | Sh | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1584 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-c-Fos Antibody |
| Alternate Names |
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| Background Information | c-fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified immunoglobulin. Lyophilized, no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL). |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-c-Fos Antibody (Sheep Polyclonal Antibody) validated in IHC, IHC(P), WB to detect c-Fos also known as G0/G1 Switch Regulatory Protein 7. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:500-1:4000, 2-8°C 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase. Recommended fixation is 4% PFA with 0.5% picric acid {Li et al 1999}; triton X-100 pretreatment in the block only; antigen retrieval for paraffin sections is recommended. Microwave citric acid treatment suggested. Western blot: 10 μg/mL. Detects a band at approximately 55 kDa. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic c-fos peptide (residues Ser.Gly.Phe.Asn.Ala.Asp.Tyr.Glu.Ala. Ser.Ser.Ser.Arg.Cys) corresponding to amino acids 4-17 of human Fos, conjugated to bovine thyroglobulin. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Sheep |
| Specificity | Recognizes c-fos. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. |
| Gene Symbol |
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| Purification Method | Ammonium Sulfate Precipitation |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P01100 # Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, c-fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SIZE: 380 amino acids; 40695 Da SUBUNIT: Heterodimer with JUN. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB (By similarity). SUBCELLULAR LOCATION: Nucleus. SIMILARITY: SwissProt: P01100 ## Belongs to the bZIP family. Fos subfamily. & Contains 1 bZIP domain. |
| Molecular Weight | 55 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized material at -20 to -70°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to one year. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the containers cap. |
| Packaging Information | |
|---|---|
| Material Size | 500 µg |