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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Eu, H, Rb, Sh | WB, IHC | Sh | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1586 |
| Replaces | 04-454 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Ubiquitin Antibody |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 mL of sterile distilled water. Centrifuge to remove any residue. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Ubiquitin using this Anti-Ubiquitin Antibody validated for use in WB, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:2,000-1:5,000. Immunoblotting: 1:1,000-1:2,000 (depending on detection system used). Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Ubiquitin from bovine red blood cells coupled to chicken gamma globulin. |
| Host | Sheep |
| Specificity | Recognizes both free ubiquitin and ubiquitin-protein conjugates on Western blots. Labels tangles and Lewy bodies in paraffin or frozen human brain sections. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimers and Down syndrome. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P62988 # Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous fonctions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. SIZE: 76 amino acids; 8565 Da SUBCELLULAR LOCATION: Cytoplasm. Nucleus. PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly. SIMILARITY: SwissProt: P62988 ## Belongs to the ubiquitin family. MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a). |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |