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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, IH(P) | Ch | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB15894 |
| Description | Anti-Tbr2 Antibody |
| Alternate Names |
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| Background Information | Eomesodermin homolog (UniProt: O54839; also known as T-box brain protein 2, T-brain-2, TBR-2, Tbr2) is encoded by the Eomes (also known as Tbr2) gene (Gene ID: 13813) in murine species. T-box genes are a family of transcription factors have a highly conserved DNA binding domain named the T-domain. Tbr2 functions as a transcriptional activator that plays a crucial role during gastrulation, mesodermal specification, and limb patterning. It plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. It is also shown to be involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Its DNA-binding region is localized to amino acids 278-458. Conditional inactivation of Tbr2 during early brain development is known to cause microcephaly and severe behavior deficits. Two isoforms of Tbr2 have been described that are produced by alternative splicing. Isoform 2 lacks amino acids 463-481. (Ref.: Bulfone, A., et al. (1999). Mech. Dev. 84 (1-2); 133-138). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Tbr2, Cat. No. AB15894, is a chicken polyclonal antibody that detects Tbr2 and is tested for use in Western Blotting and Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Tested Applications Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Tbr2 in Mouse brain tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of mouse T-box brain protein 2 (Tbr2). |
| Epitope | C-terminal |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Chicken |
| Specificity | This chicken polyclonal antibody specifically detects T-box brain protein 2 (Tbr2). It targets an epitope within 20 amino acids from the C-terminal region. |
| Isotype | IgY |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. SUBCELLULAR LOCATION: Nucleus Probable. TISSUE SPECIFICTY: Expressed in CD8+ T-cells. DEVELOPMENTAL STAGE: Originally expressed in the trophoectoderm of the blastocyst and later in the extra-embryonic ectoderm of the early post-implantation embryo. In the embryo proper, expressed in the posterior part of the epiblast. During gastrulation, extends distally into the primitive streak and nascent mesoderm. Also expressed in the developing forebrain and the olfactory lobes. Expressed at E12.5 and E14.5 in the forebrain. INDUCTION: Up-regulated in CD8+ T-cells upon activation. DISRUPTION PHENOTYPE: Embryonic lethal due to peri-implantation defects. Mutant embryos arrest soon after implantation and fail to form organized embryonic or extraembryonic structures. Conditional mutants, with expression abrogated in the inner cell mass of embryos from early implantation stages onward, display gastrulation defects. SEQUENCE SIMLARITIES: Contains 1 T-box DNA-binding domain. |
| Molecular Weight | ~72 and 74 kDa observed; 74.8 kDa calculated.. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in E13-E14 Mouse brain tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tbr2 in E13-E14 Mouse brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |