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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ELISA, ICC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1590P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Adenosine A3 Receptor Antibody, extracellular (a.a. 154-168) |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Adenosine A3 Receptor Antibody, extracellular (a.a. 154-168) is an antibody against Adenosine A3 Receptor for use in ELISA, IC & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 micrograms per mL: A3 receptors are different sizes in different species & tissues; A3R from rat brain membranes runs as a 40kDa band, 52kDa in rabbit uroepithelium membranes (Yu, W et Al. Am J. Physiol Cell Physiol 2006 in press); use proteinase inhibitors and membrane preparations for best signals. ELISA: 1:10,000-1:50,000 (50-100 ng A3R peptide (Catalog Number AG294)/well) Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Amino acid sequence corresponding to the 3rd extracellular domain (15 amino acids) of the rat A3R gene. |
| Epitope | extracellular (a.a. 154-168) |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Specific for Adenosine A3 receptor. Based on mRNA studies, the A3R has been found in rat testis and lung, kidneys, heart and brain. Other studies have found the mRNA to be highest in lung, spleen and pineal glands and moderate levels in brain kidney and testis. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a protein that belongs to the family of adenosine receptors, which are g-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P33765 # Receptor for adenosine. The activity of this receptor is mediated by g proteins which inhibits adenylyl cyclase. Possible role in reproduction. SIZE: 318 amino acids; 36185 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. PTM: Phosphorylation on Ser-317 may be crucial for rapid desensitization (By similarity). SIMILARITY: SwissProt: P33765 ## Belongs to the g-protein coupled receptor 1 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |