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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ELISA, IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1598P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Vesicular Monoamine Transporter 2 Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Liquid in PBS with 0.1% BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Vesicular Monoamine Transporter 2 Antibody is an antibody against Vesicular Monoamine Transporter 2 for use in ELISA, IH & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL (Chemiluminescence technique) Immunohistochemistry: 1-20 μg/mL on paraformaldehyde fixed sections. ELISA: 0.5-1.0 μg/mL (1 μg/mL VMAT1 peptide (Catalog Number AG251)/well) Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | A 13 amino acid C-terminal peptide sequence from rat VMAT2 (Liu et al. 1992). This peptide is predicted to be cytoplasmic. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes Vesicular Monoamine Transporter 2 (VMAT2). An antibody made to the C-terminal VMAT2 peptide detected a major band at ~55 kDa in postnuclear supernatants of CHO transfected with VMAT2 and not in wild type cells (Peter et al. 1995; Nirenberg et al. 1995). Some additional bands, both higher and lower molecular weight, were also detected and remain unaffected by the inclusion of protease inhibitors. Higher molecular weight bands may represent glycosylated VMAT2 (Peter et al. 1995; Nirenberg et al. 1995, Liu et al. 1994). VMAT2 has been localized in adrenal chromaffin cells, sympathetic ganglion cells, enterochromaffin-like cells of the stomach and in CNS (Peter et al. 1995; Nirenberg et al. 1995; Nirenberg et al. 1996). May recognize human due to the 92% homology between rat and human VMAT2. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The vesicular monoamine transporter acts to accumulate cytosolic monoamines into synaptic vesicles, using the proton gradient maintained across the synaptic vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine (Peter et al., 1993 [PubMed 7905859]). See also SLC18A1 (MIM 193002).[supplied by OMIM] |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q05940 # Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis. SIZE: 514 amino acids; 55713 Da SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: Q05940 ## Belongs to the major facilitator superfamily. Vesicular transporter family. |
| Molecular Weight | 55 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |