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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, IHC, IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1603 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Phosphoserine Antibody |
| Background Information | Cellular responses to a variety of extracellular signals occur through phosphorylation or dephosphorylation of intracellular proteins. Abnormal protein phosphorylation may be involved in the progression of numerous diseases, including many forms of cancer, immune system dysfunction and cardiovascular disease. Such changes in protein phosphorylation may be detected by immunocytochemical mapping of cells labeled with anti-phosphoserine or anti-phosphothreonine antibodies and can be correlated with cellular, electrophysiological or behavioral state changes. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit serum in buffer containing 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Phosphoserine Antibody detects level of Phosphoserine & has been published & validated for use in ELISA, IH, IP & WB. |
| Key Applications |
|
| Application Notes | Immunoprecipitation (tissue extracts): 10-20 μg anti-pS/mg protein was used from a previous lot. Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of interest, antibody amounts should be decreased to 5 μg/500 μL of extracts. Optimal concentrations must be determined experimentally. ELISA (kinase assay): A previous lot of this anitbody was used at a 1:250-1:500 dilution. Immunohistochemistry: A previous lot of this antibody was used at a 1:50 dilution. As the antibody detects all phosphoserines, any phosphorylated serine protein or peptide can be used to block antibody staining. Typically a 10 M excess of peptide is used in Tris based buffers. Western Blot Analysis: 1:500 dilution of a previous lot Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Keyhole Limpet Hemocyanin(KLH)- conjugated phosphoserine conjugates. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes phosphoserine, peptidyl-phosphoserine, and serine-phosphorylated proteins. Does not cross react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross reactivity with free phosphothreonine. Readily reacts with known phosphoproteins such as phosvitin and alpha casein. Anti-phosphoserine is species independent in reactivity |
| Species Reactivity |
|
| Species Reactivity Note | Anti-phosphoserine is species independent. |
| Antibody Type | Polyclonal Antibody |
| Modifications |
|
| Purification Method | Phosphoserine affinity chromatography |
| Molecular Weight | Dependent upon the molecular weight of the serine phosphorylated protein being detected. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on NGF treated PC12 lysates. Western Blot Analysis: 1:500 dilution of this lot detected serine phosphorylated proteins on 10 μg of NGF treated PC12 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for up to 6 months at -20°C in undiluted aliquots from date of receipt. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 uL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the containers cap. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |