Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, IH(P), IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1607 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Phosphothreonine Antibody |
| Background Information | Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in PBS buffer, containing 50% Glycerol, 0.01% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Phosphothreonine Antibody is an antibody against Phosphothreonine for use in ELISA, IH(P), IP & WB. |
| Key Applications |
|
| Application Notes | ELISA (kinase assay): A 0.5 μg/mL concentration of a previous lot was used in ELISA. Immunoprecipitation: A previous lot of this antibody was used in IP. Immunohistochemistry (paraffin): Representative image from a previous lot. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Keyhole Limpet Hemocyanin (KLH) – conjugated phosphothreonine conjugates and Phosvitin. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | By direct competitive ELISA methodology (antigen capture) the specificity of this antibody has been determined to be phosphothreonine. Binding of Ly-pT-Gly-HRP (0.2μg/100μL/well) to an anti-phosphothreonine coated microplate (0.5μg/100μL/well) is inhibited by 95% with the addition of 2-10 μg/mL anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine, or threonine at concentrations of up to 1 mg/mL. Antibody AB1607 recognizes phosphothreonine, peptidyl-phosphothreonine, and threonine-phosphorylated proteins. |
| Species Reactivity |
|
| Species Reactivity Note | Anti-phosphothreonine is species independent |
| Antibody Type | Polyclonal Antibody |
| Modifications |
|
| Purification Method | ImmunoAffinity Purified |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on Calyculin/Okadaic Acid treated HEK293 lysate. Western Blot Analysis: 1:500 dilution of this lot detected PHOSPHOTHREONINE on 10 μg of Calyculin/Okadaic Acid treated HEK293 lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |