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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ma | ICC, IHC, IH(P), WB | Gt | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1620 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Vimentin Antibody |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Neat goat Serum. Liquid containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Vimentin Antibody detects level of Vimentin & has been published & validated for use in IC, IH, IH(P) & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:20-1:40. Shows reactivity on frozen tissue sections as well as formalin-fixed paraffin embedded tissue by immunoperoxidase and immunofluorescent assays. Immunocytochemistry Western blot Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Vimentin purified from cultured human foreskin fibroblasts. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Goat |
| Specificity | Recognizes vimentin in cultured mammalian cells and tissue sections. AB1620 specifically stains the vimentin band (58 kD) using total cell or tissue extracts in immunoblotting. No reactivity to desmin, keratin, neurofilaments or glial filaments. Shows wide cross-reactivity among mammalian species using various immunolabeling techniques. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM] |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. SIZE: 466 amino acids; 53652 Da SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein. TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitts lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family. |
| Molecular Weight | 58 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 500 µL |