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Millipore This Anti-Nitric Oxide Synthase Ii Antibody Is Validated For Use In Wb, Ic For The Detection Of Nitric Oxide Synthase Ii. - Mill (Additional S&H or Hazmat Fees May Apply)
Affinity purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% Sodium azide as a preservative.
Quality Level
MQ100
Applications
Application
This Anti-Nitric Oxide Synthase II Antibody is validated for use in WB, IC for the detection of Nitric Oxide Synthase II.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Western Blot: 5 μg/mL using chemiluminescent detection. Higher concentrations of the antibody may be required if colorimetric detection is used. Antibody dilution will also vary with the concentration of the antigen. Immunocytochemistry: Use at 2-5 μg/mL
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Synthetic peptide corresponding to amino acids 1131-1144 of mouse macrophage NOS, coupled to KLH.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
Reactive with mouse NOS-II (iNOS).
Species Reactivity
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_153292.1
NM_000625.3
Entrez Gene Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Gene Symbol
NOS2A
iNOS
HEP-NOS
INOS
NOS
NOS2
EC 1.14.13.39
UniProt Number
P35228
UniProt Summary
FUNCTION: SwissProt: P35228 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. COFACTOR: Heme group (By similarity). & Binds 1 FAD (By similarity). & Binds 1 FMN (By similarity). & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity). SIZE: 1153 amino acids; 131117 Da SUBUNIT: Homodimer. Binds SLC9A3R1. TISSUE SPECIFICITY: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. SIMILARITY: SwissProt: P35228 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at 2-8°C in undiluted aliquots for up to 6 months.
