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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB16530 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-BAFF Antibody, CT |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-BAFF Antibody, C-terminus detects level of BAFF & has been published & validated for use in WB. |
| Key Applications |
|
| Application Notes | Western blot: 0.25 to 1 μg/mL. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Rabbit anti-BAFF (C-term) polyclonal antibody was raised against a peptide corresponding to amino acids 254 to 269 of human BAFF (Moore et Al. 1999; Schneider et Al. 1999; Shu et Al. 1999; Mukhopadhyay et Al. 1999). |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BlyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-kB and c-jun N-terminal kinase) (Moore et Al. 1999; Schneider et Al. 1999; Shu et Al. 1999; Mukhopadhyay et Al. 1999). BAFF/BlyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion (Moore et Al. 1999; Schneider et Al. 1999). Two receptors for BAFF were recently identified and designated TACI and BCMA (Gross et Al. 2000). BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases (Gross et Al. 2000; Khare et Al. 2000). Like TNFalpha and TRAIL, THANK was shown to activate NF-kB and c-jun N-terminal kinase (JNK) and to induce apoptosis (Mukhopadhyay et Al. 1999). |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q9Y275 # Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. SIZE: 285 amino acids; 31223 Da SUBUNIT: Homotrimer. SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor ligand superfamily member 13b, soluble form: Secreted. TISSUE SPECIFICITY: Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T- cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. PTM: The soluble form derives from the membrane form by proteolytic processing. & N-glycosylated. SIMILARITY: SwissProt: Q9Y275 ## Belongs to the tumor necrosis factor family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |