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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB16942 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-DR5 Antibody, CT |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-DR5 Antibody, C-terminus detects level of DR5 & has been published & validated for use in WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:250 to 1:500 Whole cell lysate from HeLa or K562 cells can be used as a positive control . Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Polyclonal antibody raised against a peptide corresponding to amino acids 388-407 of human DR5 precursor (Pan et al. 1997; Sheridan et al. 1997). |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2 or KILLER by several groups independently (Pan et al. 1997; Sheridan et al. 1997; Walczak et al. 1997; MacFarlane et al. 1997; Screaton et al. 1997; Wu et al. 1997; Chaudhary et al. 1997). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracelluar death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: O14763 # Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B. SIZE: 440 amino acids; 47850 Da SUBUNIT: Homotrimer. Can interact with TRADD and RIP. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLa S3, K562, HL-60, SW480, A549 and G361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. DISEASE:SwissProt: O14763 # Defects in TNFRSF10B may be a cause of squamous cell carcinoma of the head and neck (HNSCC) [MIM:275355]. SIMILARITY: SwissProt: O14763 ## Contains 1 death domain. & Contains 3 TNFR-Cys repeats. |
| Molecular Weight | Observed MW ~ 55kDa; Calculated MW ~48kDa https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/protein-biology/western-blotting/observed-vs-calculated-molecular-weight |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |