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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB17003 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Bim Antibody, internal epitope, pan-Bim isoforms |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Immunoaffinity Purified immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Bim Antibody, internal epitope, pan-Bim isoforms is validated for use in WB for the detection of Bim. |
| Key Applications |
|
| Application Notes | Western blot: 1:1000-1:2000 Human K562 cell lysate can be used as a positive control and a 23 kDa band can be detected. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to amino acids 22-40 of human origin (OConneor et al. 1998). The sequence is identical to that of mouse and is different by one amino acid from that of rat. |
| Epitope | internal epitope, pan-Bim isoforms |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes pan-Bim isoforms. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | Function: Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis. Subunit structure: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK By similarity. Subcellular location: Endomembrane system; Peripheral membrane protein By similarity. Note: Associated with intracytoplasmic membranes By similarity. Isoform BimEL: Mitochondrion Isoform BimL: Mitochondrion Isoform BimS: Mitochondrion Isoform Bim-alpha1: Mitochondrion Tissue specificity: Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. Ref.3 Domain: The BH3 motif is required for Bcl-2 binding and cytotoxicity. Sequence similarities: Belongs to the Bcl-2 family. |
| Molecular Weight | 23 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | The undiluted antibody solution is stable for approximately 6 months when stored 2–8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |