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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Rb | WB, IP, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1735 |
| Replaces | 04-438 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Bid Antibody |
| Background Information | Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is known to interact with Bcl-2 and Bax. It has been reported that Bid induces cytochrome c release from mitochondria following cleavage by caspase-8 in response to apoptotic stimuli transmitted through cell surface death receptors. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Whole serum without preservatives |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Bid using this Anti-Bid Antibody validated for use in WB, IP, IH. |
| Key Applications |
|
| Application Notes | Western blot: Recommended antibody dilution 1:3,000 to 1:6,000. Recognizes a band migrating at approximately 22 kDa corresponding to Bid. Immunoprecipitation: Recommended antibody dilution 1:200 Immunohistochemistry: Recommended antibody dilution 1:1500 Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant full length GST-tagged mouse Bid protein |
| Host | Rabbit |
| Specificity | AB1735 recognizes Bid from human, mouse, rat and rabbit. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P55957 # The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. SIZE: 195 amino acids; 21995 Da SUBUNIT: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2 (By similarity). SUBCELLULAR LOCATION: Cytoplasm (By similarity). Mitochondrion membrane (By similarity). Note=When uncleaved, it is predominantly cytoplasmic. & BH3-interacting domain death agonist p15: Mitochondrion membrane (By similarity). Note=Translocates to mitochondria as an integral membrane protein (By similarity). & BH3-interacting domain death agonist p13: Mitochondrion membrane (By similarity). Note=Associated with the mitochondrial membrane (By similarity). & Isoform 1: Cytoplasm. & Isoform 3: Cytoplasm. & Isoform 2: Mitochondrion membrane. Note=A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively. TISSUE SPECIFICITY: Isoforms 2 and 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level). DOMAIN: SwissProt: P55957 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. PTM: TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products (By similarity). & Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -20°C for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |