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Osteonectin is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a glycoprotein with a molecular weight of 43 kDa and has calcium-binding properties.
Product Information
Format
Serum
Presentation
Rabbit serum. Lyophilized. Reconstitute with 100 μL of sterile distillled water.
Quality Level
MQ100
Applications
Application
Anti-Osteonectin Antibody is an antibody against Osteonectin for use in ELISA, IH(P).
Key Applications
ELISA
Immunohistochemistry (Paraffin)
Application Notes
ELISA (1:5,000)
Immunohistochemistry on paraffin-embedded tissue sections (1:1,000)
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
recombinant osteonectin expressed in human embryonal kidney line HEK-293.
Host
Rabbit
Specificity
Human Osteonectin (ON).
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_003118.2
Entrez Gene Summary
Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003 [PubMed 12721366]).[supplied by OMIM]
Gene Symbol
SPARC
Osteonectin
BM-40
ON
UniProt Number
P09486
UniProt Summary
FUNCTION: SwissProt: P09486 # Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. SIZE: 303 amino acids; 34632 Da SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. DEVELOPMENTAL STAGE: Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair. SIMILARITY: SwissProt: P09486 ## Belongs to the SPARC family. & Contains 1 EF-hand domain. & Contains 1 follistatin-like domain. & Contains 1 Kazal-like domain.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
