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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, WB, NEUT | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1868P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Stromal Cell-Derived Factor-1α Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Lyophilized from 0.5X PBS pH 7.2. Reconstitute with sterile distilled water. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Stromal Cell-Derived Factor-1α using this Anti-Stromal Cell-Derived Factor-1α Antibody validated for use in ELISA, WB, NEUT. |
| Key Applications |
|
| Application Notes | ELISA: 0.5 μg/mL this dilution will allow detection (in direct ELISA) of 0.2-0.4 ng/well when human SDF-1alpha is used to coat the plate. Western blot: 0.1-0.2 μg/mL, this dilution should offer a detection limit of 1.5-3.0 ng/lane, under reducing or non-reducing conditions. Optimal working dilutions must be determined by the end user. Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of human SDF-1alpha (100 ng/mL), a concentration of 2.0-0.4 ng/well of recombinant human SDF-1alpha |
| Biological Information | |
|---|---|
| Immunogen | Highly pure (>98%) recombinant human SDF-1alpha. |
| Host | Rabbit |
| Specificity | Reacts with human SCDF-1alpha |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | For background information on chemokines, see CXCL11 (SCYB11; MIM 604852). Stromal cell-derived factors 1-alpha and 1-beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF (see MIM 191160), or IL1 (see MIM 147760). The intercrines are characterized by the presence of 4 conserved cysteines which form 2 disulfide bonds. They can be classified into 2 subfamilies. In the CC subfamily, which includes beta chemokine, the cysteine residues are adjacent to each other. In the CXC subfamily, which includes alpha chemokine, they are separated by an intervening amino acid. The SDF1 proteins belong to the latter group.[supplied by OMIM] |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P48061 # Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. SIZE: 93 amino acids; 10666 Da SUBCELLULAR LOCATION: Secreted. PTM: Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. SIMILARITY: SwissProt: P48061 ## Belongs to the intercrine alpha (chemokine CxC) family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile water to a concentration of =0.2 mg/ml the antibody is stable for at least six weeks at 2-4°C. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |