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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IHC, IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB19016 |
| Replaces | 04-1150 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-MMP-9 Antibody, Catalytic domain |
| Alternate Names |
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| Background Information | All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III, and is produced by normal alveolar macrophages and granulocytes. The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing PBS, containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MMP-9 Antibody, Catalytic domain is an antibody against MMP-9 for use in IH, IP & WB. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 1:1000 dilution of a previous lot was used. Immunoprecipitation: 1:500 dilution from a previous lot was used. MMP-9 is constitutively produced by some tumor cell lines (i.e., HT1080, HL60, and U937), but not in most quiescent cells and tissues. The phorbol ester TPA stimulates production of MMP-9 in some cell types. MMP-9 levels in culture media (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, g.I. et Al. J. Biol. Chem. 267, 4583-4591. 1992). This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media. Western blot: 1:2,000. Typically 98-95 kDa pro and 92-88kDa active forms are detected as well as breakdown products under fully reduced conditions. Under non-reduced conditions additional bands may be detected because of SDS-stable TIMP and other inhibitor complexes. CC069 is recommended as a positive control. Immunohistochemistry: 1:100 dilution from a previous lot was used in frozen acetone fixed tissues. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | E. coli-expressed active rat 92 kDa type IV collagenase (catalytic domain) |
| Epitope | Catalytic domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes rat MMP-9 pro and active forms. Antibody also reacts with mouse MMP-9 and human MMP-9, other species not tested. Exhibits no cross-reactivity with other MMP family members. The antibody was generated using E. coli-expressed active rat 92 kDa type IV collagenase (catalytic domain) as an immunogen. |
| Species Reactivity |
|
| Species Reactivity Note | Rat, Human, and Mouse. Reactivity with other species has not been confirmed. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq] |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P14780 # May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. COFACTOR: Binds 2 zinc ions per subunit. & Binds 3 calcium ions per subunit. SIZE: 707 amino acids; 78427 Da SUBUNIT: Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. TISSUE SPECIFICITY: Produced by normal alveolar macrophages and granulocytes. DOMAIN: SwissProt: P14780 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. PTM: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains. MISCELLANEOUS: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status. |
| Molecular Weight | 92 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on L6 lysates. Western Blot Analysis: 1:1000 dilution of this lot detected MMP-9 on 10 µg of L6 lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |