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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | WB, IF, IH(P) | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB19026 |
| Replaces | AB19031 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-ADAM 10 Antibody, CT |
| Alternate Names |
|
| Background Information | ADAM10 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It cleaves the membrane-bound precursor of TNF-alpha at 76-Ala-|-Val-77 to its mature soluble form. It is responsible for the proteolytic release of several cell-surface proteins, including heparin binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). It contributes to the normal cleavage of the cellular prion protein and is involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicules, suggesting a vesicle-based protease activity. Tumor-necrosis factor alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNA-alpha, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10-, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloproteinase domain), was recently identified to cleave TNF-alpha, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10mRNA is expressed in a variety of human and bovine tissues. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal IgG in buffer containing PBS and 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-ADAM 10 Antibody, C-terminus is validated for use in WB for the detection of ADAM 10. |
| Key Applications |
|
| Application Notes | Western blot: 1:500 - 1:2,000 dilution. Jurkat whole cell lysate can be used as a positive control and an 85 kDa band can be detected which may represent precursor. A 60 kDa faint band was detected in some cell lines including Jurkat, which appears to be the processed mature protein. Overloading of the primary antibody can lead to multiple bands appearing in some preparations. The presence of glycosylated forms and breakdown products can lead to other bands. A prominent proform at near 80-85 is usually seen, with bands at 60, and below 50 kDa in most cases. The immunogen peptide is available (Cat. No. AG533) for performing antibody blocking studies. Optimal working dilutions must be determined by end user. Immunohistochemistry: A previous lot of this antibody was used to detect ADAM10 in formalin-fixed, paraffin-embedded renal tissue and renal cell carcinoma tissue (Gutwein, P., et al. EUROPEAN JOURNAL OF CANCER. 45(2009):478–489). Immunofluorescence: A previous lot of this antibody was used to detect ADAM10 in RCC4 (renal cell carcinoma) and A498 cells (Gutwein, P., et al. EUROPEAN JOURNAL OF CANCER. 45(2009):478–489). |
| Biological Information | |
|---|---|
| Immunogen | Raised against a peptide corresponding to amino acids 732-748 of human ADAM 10 (Rosendahl, M.S., 1997). This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM 10 by one amino acid (Pan, D., 1997; Howard, L., 1996). |
| Epitope | a.a. 732-748 of human ADAM 10 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes ADAM 10, C-terminus. |
| Species Reactivity |
|
| Species Reactivity Note | Human, mouse and bovine. Expected to react with rat based on sequence homology |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O14672 # Cleaves the membrane-bound precursor of TNF-alpha at 76-Ala- -Val-77 to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (By similarity). COFACTOR: Binds 1 zinc ion (By similarity). SIZE: 748 amino acids; 84142 Da SUBUNIT: Interacts with ephrin-A2 (By similarity). SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Intracytoplasmic membrane; Single-pass type I membrane protein. Note=Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi. TISSUE SPECIFICITY: Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver. DOMAIN: SwissProt: O14672 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. PTM: The precursor is cleaved by a furin endopeptidase (By similarity). SIMILARITY: Contains 1 disintegrin domain. & Contains 1 peptidase M12B domain. |
| Molecular Weight | 80-85 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on RAW264.7 lysates. Western Blot: 1:500 dilution of this lot detected ADAM 10 on 10 μg of RAW264.7 lysates |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |