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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, IP, WB, IHC, NEUT | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2215 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Interferon-β Antibody |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal serum in buffer containing 0.3 M NaHCO3 and 200 mM NaCl. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Interferon-β using this Anti-Interferon-β Antibody validated for use in ELISA, IP, WB, IH, NEUT. |
| Key Applications |
|
| Application Notes | Cytopathic effect inhibition assay: 2 x 10E5 neutralization units per mL of antiserum. One neutralization unit is the amount of antiserum required to neutralize 1 unit of mouse IFN-beta. This material is prepared specifically for effective neutralization of mouse IFN-beta. Western blot (1:500) The antibody reacts with a 19kDa band coresponding to Mu-IFN-beta. Protocol below: 1. Put protein (approx 50ng) in blocking solution for 30-60 min RT 2. Add Primary antibody (1:500) to blocking solution 3. Agitate membrane for 60 min, RT 4. Wash 3X for 5min each with PBS-Tween 5. Add secondary antibody (1:1000) after diluting in PBS-Tween 6. Gentle agitation for 60min. 7. Wash 3X for 5min each with PBS-Tween 8. Develop with reaction kit of choice Immunoprecipitation Immunohistochemistry ELISA Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Mouse IgG, whole molecule. |
| Host | Rabbit |
| Specificity | Reacts with mouse Interferon beta (IFNbeta). No reactivity is seen with IFNalpha or gamma |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P01574 # Has antiviral, antibacterial and anticancer activities. SIZE: 187 amino acids; 22294 Da SUBUNIT: Monomer. SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: P01574 ## Belongs to the alpha/beta interferon family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -20°C or below for retention of full activity, for up to 12 months. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |
| Material Package | 2x10(e4) units |