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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M | WB, IHC | Gp | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2251-I |
| Description | Anti-VGluT2 Antibody |
| Alternate Names |
|
| Background Information | Vesicular glutamate transporter 2 (UniProt Q9JI12; also known as Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6, VGluT2) is encoded by the Slc17a6 (also known as Dnpi, Vglut2) gene (Gene ID: 84487) in rat. VGluT2 is a multi-pass membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neurons. It contains seven cytoplasmic domains, twelve transmembrane domains, and six vesicular domains. Its N- and C-terminal tails (aa 1-71 and 499-582, respectively) are exposed at the cytoplasmic side. VGluT1- and VGluT2-expressing neurons represent the bulk of canonical glutamatergic neurons in the brain, with VGluT1 representing the major subtype and accounting for approximately 80% of total vesicular transport of glutamate in the brain. Expression of VGluT2 is reported to increase sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGluT1 and VGluT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGluT2 expression predominates. VGluT1 expression becomes essential for survival at the time of the developmental switch from VGLUT2 to VGLUT1. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Guinea Pig polyclonal antibody in buffer containing serum with 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-VGluT2, Cat. No. AB2251-I is a Guinea pig polyclonal antibody that detects VGluT2 and is tested for use in Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:2,000 dilution from a representative lot detected VGluT2 in mouse brain and rat brain tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal, cytoplasmic domain of rat Vesicular Glutamate Transporter 2 (VGluT2). |
| Epitope | C-terminus. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Guinea Pig |
| Specificity | This Guinea Pig polyclonal antibody detects Vesicular glutamate transporter 2. It targets an epitope within 18 amino acids from the C-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Rat, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Unpurified. |
| UniProt Number |
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| Molecular Weight | ~56 kDa observed; 64.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Rat Brain Membrane tissue lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected VGluT2 in Rat Brain Membrane tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |