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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Pm, Chp | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2959 |
| Replaces | AB19025 |
| Description | Anti-ADAM 9 Antibody, disintegrin domain |
| Alternate Names |
|
| Background Information | A disintegrin and metalloproteinase domain-containing protein 9 (ADAM 9) belongs to a family of transmembrane, membrane-anchored, disintegrin-containing proteins that are involved in several biological functions such as protein ectodomain shedding, cell fusion, and cell adhesion. ADAMs contain several characteristic domains, two of which, the protease and disintegrin domains, are noted for their homology to snake venom metalloprotease. The exact function of ADAM 9 is yet unknown, however it is widely distributed, is found within keratinocytes, and is ideally placed on the cell membrane, which some studies have suggested supports a role in cell migration and motility. Alternative splicing results in two isoforms; isoform 1 is found within the cell membrane and is considered a single-pass type 1 membrane protein, while isoform 2 is secreted. Mutations in ADAM 9 expression are causal to cone-rod dystrophy type 9 or CORD9 which is characterized by visible retinal pigment deposits and loss of cone photoreceptors leading and rod degeneration, ultimately resulting in severe loss of vision. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mm NaCl, with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect ADAM 9 using this Anti-ADAM 9 Antibody, disintegrin domain validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the disintegrin domain of human ADAM 9. |
| Epitope | disintegrin domain |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human. Predicted to react with primate and chimpanzee based on 100% sequence homology. Mouse and rat (90% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Two alternative splice variants have been identified, encoding distinct isoforms. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP. COFACTOR: Binds 1 zinc ion per subunit (Probable). SIZE: 819 amino acids; 90556 Da SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. TISSUE SPECIFICITY: Widely expressed. Expressed in chondrocytes. Highest expression of isoform 2 found in liver and heart. SIMILARITY: Contains 1 disintegrin domain. & Contains 1 EGF-like domain. & Contains 1 peptidase M12B domain. |
| Molecular Weight | ~ 90 kDa observed. 2 isoforms are produced by alternative splicing: Isoform 1 at 91 kDa and Isoform 2 at 72 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human chondrocyte cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected ADAM 9 in 10 µg of human chondrocyte cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |