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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ht, M, Mk, R | ICC, IF, IHC, IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3000 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-IP3 Receptor Type II Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified versus immobilized immunogen peptide. Liquid in PBS buffer, containing 0.05% BSA, 10% glycerol, 0.005% sodium azide. Caution: Sodium azide is hazardous. MSDS available upon request. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-IP3 Receptor Type II Antibody detects level of IP3 Receptor Type II & has been published & validated for use in IC, IF, IH, IP & WB. |
| Key Applications |
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| Application Notes | Western blot: 1:50 - 1:100 (detects a 260 kDa band) Immunofluorescence: 1:10 - 1:20 Immunoprecipitation Immunocytochemistry: Known to give positive reactivity on AR4-2J, HL-60, COS-I, HEK-293, and RBL-2H3 cell lines. Immunohistochemistry: Paraformaldehyde or methanol fixes are effective. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to the carboxy terminus of the rat Type II receptor |
| Host | Rabbit |
| Specificity | Rabbit anti-Type II Inositol 1,4,5-Trisphosphate Receptor (IP3R) is directed against a synthetic peptide corresponding to the carboxy terminus of the rat Type II receptor. The antibody is reactive with Type II IP3R from rat, mouse, human, hamster, and monkey. |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q14571 # Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. SIZE: 2701 amino acids; 308078 Da SUBUNIT: Homotetramer (By similarity). SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: The short isoform is found in skeletal muscle and heart. DOMAIN: SwissProt: Q14571 The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand- binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. PTM: Phosphorylated on tyrosine residues (By similarity). SIMILARITY: Belongs to the InsP3 receptor family. & Contains 5 MIR domains. MISCELLANEOUS: Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium- binding protein which then inhibits the receptor. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -20°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |