Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ELISA, IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3066 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Aquaporin 2 Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin.Liquid in 100 mM Tris, pH 7.5 with 0.2% BSA and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Aquaporin 2 using this Anti-Aquaporin 2 Antibody validated for use in ELISA, IH & WB. |
| Key Applications |
|
| Application Notes | Western Blotting: 1-10 μg/mL for affinity pure antibody using Chemiluminescence technique. An antibody made to the AQP2 peptide detected a major band at ~29 kDa in rat kidney cortex, and 35-47 kDa in inner and outer medulla (postranslational or glycosylation) (Nielsen et al. 1993; Deen et al. 1995). A similar pattern is seen in the human kidney (Sasaki, et al. 1994). Immunohistochemistry: 2-10 μg/mL in paraformaldehyde fixed sections of tissues. AQP2 has been localized in apical membranes (Fushimi et al. 1993; Nielsen et al. 1993; Deen et al. 1995). ELISA: 0.5-1.0 μg/mL using 1 μg/mL control peptide/well. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | A 15 aa C-terminal peptide from rat AQP2. This peptide is predicted to be cytoplasmic. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Aquaporin 2 (AQP-CD/WCH-CD). SPECIES REACTIVITY: The antibody has not yet been tested for cross-reactivity to other species. The 15 aa C-terminal peptide has 100% homology with mouse and 93% homology with human AQP2. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant, and recessive forms of nephrogenic diabetes insipidus. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P41181 # Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. SIZE: 271 amino acids; 28837 Da SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note=Shuttles from vesicles to the apical membrane. TISSUE SPECIFICITY: Expressed in renal collecting tubules. DOMAIN: SwissProt: P41181 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). PTM: Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. DISEASE:SwissProt: P41181 # Defects in AQP2 are a cause of autosomal nephrogenic diabetes insipidus, type I (AD-NDI) [MIM:125800]. It is characterized by excessive water drinking (polydypsia) and urine excretion (polyuria). SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |