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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Ma, Pl, Su | ICC, IH(P), WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3201 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Tubulin Antibody, Detyrosinated |
| Alternate Names |
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| Background Information | Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing liquid PBS. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Tubulin Antibody, Detyrosinated is an antibody against Tubulin for use in IC, IH(P) and WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: A previous lot was used on either frozen or paraffin embedded, fixed tissue (Gundersen, G.G., 1984): 1:200-1:500 dilution. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Peptide corresponding to the C-terminal of Glu tubulin. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The antibody specifically recognizes the detyrosinated form (Glu tubulin) of the tubulin alpha chain (Bianchi, M., 2006; Goulimari, P., 2005). |
| Species Reactivity |
|
| Species Reactivity Note | Human, mouse, and rat. Expected to cross-react with mammals, sea urchin, plants, not yeast (Paturle-Lafanechere, L., 1991). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Non-Reactive Species |
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| Purification Method | Affinity purified immunoglobulin |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE: 450 amino acids; 50433 Da SUBUNIT: Dimer of alpha and beta chains. DOMAIN: SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium. SIMILARITY: Belongs to the tubulin family. |
| Molecular Weight | 50 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on L6 lysate lysates. Western Blot Analysis: 1:500 dilution of this lot detected detyrosinated tubulin on 10 μg of L6 lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |