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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ma, Pl, Ech, Su | IH(P), WB, ICC, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3203 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Tubulin Antibody, δ 2 |
| Background Information | Tubulin, the dimeric subunit of microtubules, is submitted to post translational modifications including cyclic tyrosine removal and addition at the COOH terminus of the alpha subunit. The cycle involves two enzymes, TTL (Tubuline Tyrosine Ligase) and Tubulin carboxypeptidase, and generates two major forms: tyrosinated tubulin and detyrosinated tubulin (Glu-tubulin). Removal of the COOH-terminal Glu residue from the alpha chain of Glu-tubulin generates the delta2-tubulin form. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in PBS. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Tubulin Antibody, δ 2 is validated for use in IH(P), WB, IC, IH for the detection of Tubulin. |
| Key Applications |
|
| Application Notes | Western blot: 1:1,000 Immunohistochemistry and EM on either frozen or paraffin embedded, fixed tissue (Mialhe et al. 2001): 1:500 Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Peptide corresponding to the C-terminal of delta 2 tubulin. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The antibody specifically recognizes the delta 2 form of the tubulin alpha chain (Paturle-Lafanechere et al. 1991). SPECIES REACTIVITY: Mammals, sea urchin, plants, not yeast (Lafanechere et al. 1994). |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Non-Reactive Species |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q9UJT1 # In the elongating spermatid it is associated with the manchette, a specialized microtubule system present during reshaping of the sperm head (By similarity). SIZE: 453 amino acids; 51034 Da SUBCELLULAR LOCATION: Centrosome, centriole. Cytoplasm. Nucleus. Note=Associated with centrioles. Both cytoplasmic and nuclear. SIMILARITY: SwissProt: Q9UJT1 ## Belongs to the tubulin family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |