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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ma | ELISA, RIA | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3244 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-GLP-1 (7-36) Antibody, CT amide-reactive |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit serum, diluted. Liquid in PBS containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GLP-1 (7-36) Antibody, C-terminus amide-reactive detects level of GLP-1 (7-36) & has been published & validated for use in ELISA, RIA. |
| Key Applications |
|
| Application Notes | RIA: 1:300,000. Ka = 0.8 x 10E12 L/mol for GLP-1(7-36) amide. Detection limit 0.1 fmol/tube, 95% confidence limits. EIA (capture). Detection limit 1.5 pmol/liter. Note: Plasma samples require extraction for RIA, but not for sandwich EIA. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Whole synthetic peptide. |
| Epitope | C-terminus amide-reactive |
| Host | Rabbit |
| Specificity | Glucagon-like peptide-1(7-36) amide (GLP-1), C-terminal reactive. Cross-reacts fully with GLP-1(1-36) amide but shows <0.1% cross reactivity with the unamidated forms GLP-1(7-37) and GLP-1(1-37) or other related peptides such as hGLP-2, glucagon, hGIP and VIP. SPECIES REACTIVITIES: Most mammalian species. GLP-1 is identical in all mammalian species studied except guinea pig and presumably its South American rodent relatives. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific g-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. |
| Gene Symbol |
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| Non-Reactive Species |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P01275 # Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life. SIZE: 180 amino acids; 20909 Da SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. PTM: Proglucagon is post-translationally processed in a tissue- specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas. SIMILARITY: SwissProt: P01275 ## Belongs to the glucagon family. MISCELLANEOUS: In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |