Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ICC, IHC, WB | Sh | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3265 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Actin γ Antibody |
| Background Information | Actin is a 43kDa highly conserved structural protein. There are three main actin isotypes (alpha,beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. {Pardo et al, 1983, Cell 32:1093-1103 & Otey, CA et al 1986 J Cell Biol 102:1726-1737, Bassell et al, 1998, J Neurosci 18:251-265 {available online}. Furthermore, there are at least six actin isoforms in vertebrates: cardiac alpha-actin and skeletal-alpha actin (expressed in striated muscle), vascular alpha-actin and enteric gamma-actin (expressed in smooth muscle), and cytoplasmic beta- and gamma-actins.{http://www.cbil.upenn.edu/MTIR/gammaActin_E-toc.html} |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Serum. Liquid, containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Actin Antibody, γ is an antibody against Actin for use in IC, IH & WB. |
| Key Applications |
|
| Application Notes | Western Blot: 1:500-1:2,000 or greater; non-specific bands can occur when primary antibody is overloaded. Blocking with purified milk proteins preferred over commerical concentrated milk solutions. Detects a 42-43kDa band. Specificity for gamma actin determined by 2D gel electrophoresis. Immunocytochemistry: 1:500-1:2,000. Cultured cells fixed with PFA, then permeabilized with methanol. Immunohistochemistry: 1:500-1:2,000. Tissues frozen and then fixed with PFA followed by Triton permeabilisation. 10% buffered formalin fixed tissues have also been used. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to the amino terminus of gamma actin {EEEIAALVIDNGSGGSG} conjugated to KLH. |
| Epitope | gamma |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Sheep |
| Specificity | Recognizes actin, gamma. Specificity for gamma actin determined by 2D gel electrophoresis. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P63261 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. SIZE: 375 amino acids; 41793 Da SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. DISEASE: SwissProt: P63261 # Defects in ACTG1 are the cause of non-syndromic sensorineural deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. SIMILARITY: SwissProt: P63261 ## Belongs to the actin family. MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. |
| Molecular Weight | 42-43kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |