The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
ATM/ATR-substrate CHK2-interacting zinc finger protein
ASCIZ
Zinc finger protein 822
Background Information
ASCIZ is a transcription factor that interacts with the potent kinase ATM and regulates its activity and stability. ATM is best known for its role as an apical activator of the DNA damage response in the face of DNA double-strand breaks (DSBs). Following induction of DSBs, ATM mobilizes one of the most extensive signaling networks that responds to specific stimuli and modifies directly or indirectly a broad range of targets. ASCIZ plays a crucial role in cell survival and RAD51 foci formation in response to methylating DNA damage as well. ASCIZ is also critical in development. Knockouts are lethal at E16 and show severe organ developmental defects, including a complete absence of lungs similar to mutants in Wnt2-2b/ß-catenin and FGF10/FGFR2b signaling pathways. Thus, ASCIZ has dual functions as an efficiency factor for DNA base damage repair as well as a key transcriptional regulator of early lung development. ASCIZ is localized to the nucleus in discrete foci during G1 and is ubiquitously expressed in normal and cancerous tissues.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-ASCIZ is an antibody against ASCIZ for use in Western Blotting.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human ASCIZ required for formation of RAD51 foci.
Epitope
Required for formation of RAD51 foci
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Mouse
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_056066
Gene Symbol
ATMIN
KIAA0431
ZNF822
Purification Method
Affinity Purfied
UniProt Number
O43313
Molecular Weight
~100/120 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human skeletal muscle tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ASCIZ in 10 µg of human skeletal muscle tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
