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Millipore This Anti-Myosin Light Chain Antibody, Phospho-Specific (Ser19) Is Validated For Use In Elisa, Wb For The Detection Of Myosin Light Chain. - Mill (Additional S&H or Hazmat Fees May Apply)
Affinity purified immunoglobulin. Liquid in 0.02M Potassium phosphate, pH 7.2 with 0.15 M Sodium chloride and 0.01% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Myosin Light Chain Antibody, phospho-specific (Ser19) is validated for use in ELISA, WB for the detection of Myosin Light Chain.
Key Applications
ELISA
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Western blot: 1:1,000-1:5,000 using ECL. ELISA: 1:120,000-1:135,000 Immunohistochemistry: 2.5 μg/mL
Biological Information
Immunogen
Synthetic phosphopeptide from human myosin, smooth and non-muscle regulatory light chain.
Epitope
phospho-specific (Ser19)
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
Myosin, human, smooth and non-muscle regulatory light chain, phosphoSer19. By Western blot the antibody recognizes the ~20 kDa phosphorylated protein. The immunogen sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain. The antibody recognizes monophosphorylated (pSer19) and diphosporylated (pThr18-pSer19) forms of the protein. Reactivity to non-phosphorylated human myosin, smooth and non-muscle regulartory light chain is minimal (less than 1%) by ELISA.
Species Reactivity
Human
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_002475.3
Entrez Gene Summary
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in both slow-twitch skeletal muscle and in nonmuscle tissue.
Gene Symbol
MYL6B
MLC1SA
MLC1sa
Modifications
Phosphorylation
UniProt Number
P14649
UniProt Summary
FUNCTION: SwissProt: P14649 # Regulatory light chain of myosin. Does not bind calcium. SIZE: 208 amino acids; 22764 Da SUBUNIT: Myosin is an hexamer of 2 heavy chains and 4 light chains. SIMILARITY: SwissProt: P14649 ## Contains 3 EF-hand domains.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
