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Millipore Detect Sp-A Using This Rabbit Polyclonal Antibody, Anti-Surfactant Protein A Antibody Validated For Use In Western Blotting & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Pulmonary surfactant-associated protein A1 (SP-A1, SFTPA1, PSP-A, SP-A) binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. There are four main surfactant proteins, known as SP-A, B, C, and D. SP-A was the first surfactant protein to be identified, and is also the most abundant. Its molecular mass varies from 26-38 kDa. It can be found in an open or closed form depending on the other substances present in the system. Calcium ions produce the closed-bouquet form. SP-A is necessary for the production of tubular myelin, a lipid transport structure unique to the lungs. Genetic variations in SP-A are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF) and respiratory distress syndrome in premature infants (RDS).
Product Information
Format
Purified
Control
Human lung tissue lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect SP-A using this rabbit polyclonal antibody, Anti-Surfactant Protein A Antibody validated for use in western blotting & IHC (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Surfactant Protein A in human lung tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-type lectin domain of human Surfactant Protein A.
Epitope
C-type lectin domain
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the C-type lectin domain of Surfactant Protein A.
Species Reactivity
Human
Mouse
Species Reactivity Note
Demonstrated to react with Human and Mouse. Predicted to react with Primate based on 100% sequence homology. Other homologies: Uniprot shows no homology to Rat.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_005402
Gene Symbol
SFTPA1
COLEC4
PSAP
SFTP1
SFTPA
SFTPA1B
Purification Method
Protein A Purfied
UniProt Number
Q8IWL2
Molecular Weight
~35 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human lung tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Surfactant Protein A in 10 µg of human lung tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
