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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ELISA, IHC, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3430 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Prosurfactant Protein B Antibody, reacts with both CT and NT |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit serum. Liquid. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Prosurfactant Protein B using this Anti-Prosurfactant Protein B Antibody, reacts with both C-terminus & N-terminus validated for use in ELISA, IH & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:4,000-1:8,000 on human tissue with and without antigen retrieval (see protocol on back) and 1:1,000-1:2,000 on adult and fetal mouse lung (see notes on back). Immunoblot: 1:1,000-1:5,000; proprotein 42-46kDa. ELISA: 1:500-1:1,000 Optimal dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Full length recombinant peptide (381 a.a.) of the human SP-B (pre)proprotein. |
| Epitope | reacts with both C-terminus and N-terminus |
| Host | Rabbit |
| Specificity | Reacts strongly with human and mouse proSP-B (detects both the N-terminal and C-terminal propeptides). Does not react with mature SP-B. On human tissue immunostains alveolar Type II cells and non-ciliated bronchiolar cells. On mouse tissue immunostains alveolar Type II cells and non-ciliated bronchiolar (Clara cells) in distal and terminal airways. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The SFTPB gene encodes the pulmonary-associated surfactant B protein (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Surfactant is composed of phospholipids and other surfactant-associated proteins (Clark et Al., 1995 [PubMed 7644495]). See also SFTPA1 (MIM 178630), SFTPC (MIM 178620), and SFTPD (MIM 178635).[supplied by OMIM] |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P07988 # Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter. SIZE: 381 amino acids; 42117 Da SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted, extracellular space. DISEASE: SwissProt: P07988 # Defects in SFTPB are the cause of pulmonary surfactant metabolism dysfunction type 1 (SMDP1) [MIM:265120]; also called pulmonary alveolar proteinosis due to surfactant protein B deficiency. Inborn errors of pulmonary surfactant metabolism are genetically heterogeneous disorders resulting in severe respiratory insufficiency or failure in full-term neonates or infants. These disorders are associated with various pathologic entities, including pulmonary alveolar proteinosis (PAP), desquamative interstitial pneumonitis (DIP), or cellular non- specific interstitial pneumonitis (NSIP). SIMILARITY: SwissProt: P07988 ## Contains 1 saposin A-type domain. & Contains 3 saposin B-type domains. MISCELLANEOUS: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C). |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |