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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ELISA, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3500P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Anion Exchanger 1 Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in Liquid in PBS with 0.1% BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Anion Exchanger 1 Antibody detects level of Anion Exchanger 1 & has been published & validated for use in ELISA & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL using ECL. AE1 has a molecular weight of approximately 90-100 kDa. It aggregates easily forming dimers and multimers. It may also be proteolytically processed to smaller fragments of 60, 40 and 20 kDa. ELISA: 1-10 μg/mL using 1 μg/mL control peptide per well. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | A 20 amino acid peptide sequence near the N-terminus of rat AE1 (Kudrycki et Al. 1989; Kopito et Al. 1985; Raida et Al. 1989; Lux et Al. 1989; Tanner et Al. 1988). |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes rat Anion Exchanger 1 (AE1). The immunogen shows no significant sequence homology with other AE or other proteins. SPECIES REACTIVITIES: The immunogen sequence is 90% conserved in mouse, 75% in human and 70% in bovine. Reactivity with other species has not been confirmed. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P02730 # Band 3 is the major integral glycoprotein of the erythrocyte membrane. Band 3 has two functional domains. Its integral domain mediates a 1:1 exchange of inorganic anions across the membrane, whereas its cytoplasmic domain provides binding sites for cytoskeletal proteins, glycolytic enzymes, and hemoglobin. SIZE: 911 amino acids; 101792 Da SUBUNIT: A dimer in solution, it spans the membrane asymmetrically and appears to be tetrameric. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Erythrocytes. PTM: Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1- resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation. & Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation. DISEASE: SwissProt: P02730 # Defects in SLC4A1 are the cause of elliptocytosis 4 (EL4) [MIM:109270]. Elliptocytosis (also known as ovalocytosis) is a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Several abnormalities in the membrane skeleton have been identified, including a number that have been localized to spectrin. Elliptocytosis due to defects in SLC4A1 is also referred to as rhesus-unlinked elliptocytosis [MIM:130600]. & Defects in SLC4A1 are a cause of hereditary spherocytosis (HS) [MIM:109270]. HS is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. & Defects in SLC4A1 are a cause of familial distal renal tubular acidosis (dRTA) [MIM:179800]. This disease is characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Inheritance is generally autosomal dominant, but recessive forms have also been reported. SIMILARITY: SwissProt: P02730 ## Belongs to the anion exchanger (TC 2.A.31) family. MISCELLANEOUS: Phenyl isothiocyanate inhibits anion transport in vitro. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |